Issue 83, 2019

Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs

Abstract

Herein we demonstrate the first example of oligonucleotide-templated reaction (OTR) performed on paper, using lateral flow to capture and concentrate specific nucleic acid biomarkers on a test line. Quantitative analysis, using a low-cost benchtop fluorescence reader showed very high specificity down to the single nucleotide level and proved sensitive enough for amplification-free, on-chip, detection of endogenous concentrations of miR-150-5p, a recently identified predictive blood biomarker for preterm birth.

Graphical abstract: Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs

Associated articles

Supplementary files

Article information

Article type
Communication
Submitted
19 Jul 2019
Accepted
16 Sep 2019
First published
17 Sep 2019
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2019,55, 12451-12454

Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs

S. Pavagada, R. B. Channon, J. Y. H. Chang, S. H. Kim, D. MacIntyre, P. R. Bennett, V. Terzidou and S. Ladame, Chem. Commun., 2019, 55, 12451 DOI: 10.1039/C9CC05607F

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