SCHEDULED MAINTENANCE
Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography†
João H. P. M.
Santos, 
ab
Ana M.
Ferreira,
a
Mafalda R.
Almeida,
a
Paula S. G. N.
Quinteiro,
c
Ana C. R. V.
Dias,
c
João A. P.
Coutinho,
a
Mara G.
Freire,
a
Carlota O.
Rangel-Yagui
*b
and
Sónia P. M.
Ventura
*a
ab
Ana M.
Ferreira,
a
Mafalda R.
Almeida,
a
Paula S. G. N.
Quinteiro,
c
Ana C. R. V.
Dias,
c
João A. P.
Coutinho,
a
Mara G.
Freire,
a
Carlota O.
Rangel-Yagui
*b
and
Sónia P. M.
Ventura
*a
Abstract
Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (∼100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units.
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