Issue 6, 2019

Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

Abstract

Molecular imaging of targeted large biomolecules has been restricted in SIMS due to the limited number of probes containing SIMS-detectable isotopes. We introduce here new 19F-containing molecules that can be conjugated in a site-specific manner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs). In this work, we demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens.

Graphical abstract: Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

Supplementary files

Article information

Article type
Communication
Submitted
31 Mar 2019
Accepted
24 Apr 2019
First published
24 Apr 2019
This article is Open Access
Creative Commons BY license

J. Anal. At. Spectrom., 2019,34, 1083-1087

Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry

S. Kabatas, P. Agüi-Gonzalez, R. Hinrichs, S. Jähne, F. Opazo, U. Diederichsen, S. O. Rizzoli and N. T. N. Phan, J. Anal. At. Spectrom., 2019, 34, 1083 DOI: 10.1039/C9JA00117D

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