Issue 46, 2019, Issue in Progress

Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR

Abstract

Sensitivity enhancement by isotope enrichment and DNP NMR enables detection of minor but biologically relevant species in native intact bone, including nucleic acids, choline from phospholipid headgroups, and histidinyl and hydroxylysyl groups. Labelled matrix from the aggressive osteosarcoma K7M2 cell line confirms the assignments of nucleic acid signals arising from purine, pyrimidine, ribose, and deoxyribose species. Detection of these species is an important and necessary step in elucidating the atomic level structural basis of their functions in intact tissue.

Graphical abstract: Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR

Supplementary files

Article information

Article type
Paper
Submitted
29 Apr 2019
Accepted
08 Aug 2019
First published
27 Aug 2019
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 26686-26690

Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR

I. Goldberga, R. Li, W. Y. Chow, D. G. Reid, U. Bashtanova, R. Rajan, A. Puszkarska, H. Oschkinat and M. J. Duer, RSC Adv., 2019, 9, 26686 DOI: 10.1039/C9RA03198G

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