Bioassay-guided purification of sesquiterpenoids from the fruiting bodies of Fomitopsis pinicola and their anti-inflammatory activity

Shih-Huang Tai; Ping-Chung Kuo; Ching-Che Hung; Ying-Hsuan Lin; Tsong-Long Hwang; Sio Hong Lam; Daih-Huang Kuo; Jin-Bin Wu; Hsin-Yi Hung; Tian-Shung Wu

doi:10.1039/C9RA05899K

Bioassay-guided purification of sesquiterpenoids from the fruiting bodies of Fomitopsis pinicola and their anti-inflammatory activity†

Shih-Huang Tai,‡^a Ping-Chung Kuo,‡^b Ching-Che Hung,^c Ying-Hsuan Lin,

^d Tsong-Long Hwang,

^e Sio Hong Lam,^b Daih-Huang Kuo,^f Jin-Bin Wu,^g Hsin-Yi Hung*^b and Tian-Shung Wu

*^bf

Author affiliations

* Corresponding authors

^a Departments of Surgery and Anesthesiology, Institute of Biomedical Engineering, National Cheng Kung University, Medical Center and Medical School, Tainan 701, Taiwan

^b School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan
E-mail: tswu@mail.ncku.edu.tw, z10308005@email.ncku.edu.tw
Fax: +886-6-2740552
Tel: +886-6-2747538

^c Department of Chemistry, National Cheng Kung University, Tainan 70101, Taiwan

^d Department of Environmental Sciences, University of California, Riverside, California 92521, USA

^e Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Research Center for Industry of Human Ecology, Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan

^f Department of Pharmacy, College of Pharmacy and Health Care, Tajen University, Pingtung 907, Taiwan

^g Jen-Li Biotechnology Company, Taichung, Taiwan

Abstract

Twelve undescribed sesquiterpenoids, fomitopins A–L (1–12), were isolated via bioassay-guided purification from the bracket fungus Fomitopsis pinicola (Sw.) P. Karst, and this fungus have been reported to exhibit anti-microbial and anti-inflammatory activities. The structures of 1–12 were elucidated by spectroscopic and spectrometric analyses and their absolute configurations were further confirmed by ECD simulations. Ten isolated compounds were evaluated for their anti-inflammatory potential and compound 11 exhibited the most significant inhibition of superoxide anion generation and elastase release with IC₅₀ values of 0.81 ± 0.15 and 0.74 ± 0.12 μM. These newly purified sesquiterpenoids could be potential candidates for further anti-inflammatory studies.

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Bioassay-guided purification of sesquiterpenoids from the fruiting bodies of Fomitopsis pinicola and their anti-inflammatory activity†

Abstract

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Bioassay-guided purification of sesquiterpenoids from the fruiting bodies of Fomitopsis pinicola and their anti-inflammatory activity

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