Issue 11, 2020

Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions

Abstract

Directed guidance of neurites is a pre-requisite for tailor-made designs of interfaces between cells and semiconducting components. Grayscale lithography, reactive ion etching, and ultraviolet nanoimprint lithography are potent semiconductor industry-compatible techniques for a cost- and time-effective fabrication of modulated surfaces. In this work, neurite outgrowth of murine cerebellar neurons on 2.5D pathways produced with these methods is studied. Structures of micron-sized steps and grooves serve as cell culture platforms. The effects of contact guidance through topography and chemical guidance through selective poly-D-lysine coating on these platforms are analyzed. As a consequence, the herein presented fabrication approach can be utilized to cultivate and to study low-density neuronal networks in 2.5D configuration with a high degree of order.

Graphical abstract: Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions

Supplementary files

Article information

Article type
Paper
Submitted
23 Mar 2020
Accepted
14 Jul 2020
First published
14 Jul 2020
This article is Open Access
Creative Commons BY license

Nanoscale Adv., 2020,2, 5192-5200

Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions

C. Fendler, J. Harberts, L. Rafeldt, G. Loers, R. Zierold and R. H. Blick, Nanoscale Adv., 2020, 2, 5192 DOI: 10.1039/D0NA00549E

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