Issue 33, 2020

Photoswitchable dynasore analogs to control endocytosis with light

Abstract

The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single-wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.

Graphical abstract: Photoswitchable dynasore analogs to control endocytosis with light

Associated articles

Supplementary files

Article information

Article type
Edge Article
Submitted
13 Jul 2020
Accepted
13 Aug 2020
First published
17 Aug 2020
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2020,11, 8981-8988

Photoswitchable dynasore analogs to control endocytosis with light

N. Camarero, A. Trapero, A. Pérez-Jiménez, E. Macia, A. Gomila-Juaneda, A. Martín-Quirós, L. Nevola, A. Llobet, A. Llebaria, J. Hernando, E. Giralt and P. Gorostiza, Chem. Sci., 2020, 11, 8981 DOI: 10.1039/D0SC03820B

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