Issue 30, 2020

Single-molecule DNA origami aptasensors for real-time biomarker detection

Abstract

Here we report the use of DNA nanostructures as platforms to monitor the inherent conformational changes of aptamers upon analyte binding, with single-molecule resolution and real-time capability. An aptasensor designed to sense cortisol was found to suffer from instability in solution, but this was reconciled via a rational design of a single-molecule sensing platform. In this regard, DNA origami was employed to immobilise individual aptasensors on a glass surface and to ensure adequate interaction with their environment, for single-molecule analysis. The strategy presented here can be applied to any aptamer obtained by the destabilisation of a duplex in a SELEX process, and hence employed in the rational design of single-molecule biosensors.

Graphical abstract: Single-molecule DNA origami aptasensors for real-time biomarker detection

Supplementary files

Article information

Article type
Communication
Submitted
19 May 2020
Accepted
17 Jul 2020
First published
17 Jul 2020
This article is Open Access
Creative Commons BY-NC license

J. Mater. Chem. B, 2020,8, 6352-6356

Single-molecule DNA origami aptasensors for real-time biomarker detection

K. Cervantes-Salguero, M. Freeley, J. L. Chávez and M. Palma, J. Mater. Chem. B, 2020, 8, 6352 DOI: 10.1039/D0TB01291B

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