Issue 7, 2012
From the journal:

Analytical Methods

Bovine serum albumin-coated quantum dots as a cytoplasmic viscosity probe in a single living cell†

Yuko Nakane,a   Akira Sasaki,b   Masataka Kinjob  and  Takashi Jin*ac  

* Corresponding authors

a Laboratory for Nano-Bio Probes, Quantitative Biology Center, Riken, Furuedai 6-2-3, Suita, Osaka 565-0874, Japan
E-mail: tjin@riken.jp
Fax: +81 6-6155-0112
Tel: +81 70-6800-3896

b Faculty of Advanced Life Science, Hokkaido University, Kita-21 Nishi-11 Kita-ku, Sapporo 001-0021, Japan
Fax: +81 11-706-9045
Tel: +81 11-706-9005

c WPI Immunology Frontier Research Center, and Graduate School of Frontier Biosciences, Osaka University, 1-3, Yamadaoka, Suita, Osaka, Japan

Abstract

We report that bovine serum albumin (BSA)-coated quantum dots can be used as a fluorescent probe for measuring the cytoplasmic viscosity of a single living cell: fluorescence correlation spectroscopy measurements for a HeLa cell incorporating BSA-quantum dots show that the viscosity of cytoplasm is 4.7 times greater than that of water.

Graphical abstract: Bovine serum albumin-coated quantum dots as a cytoplasmic viscosity probe in a single living cell

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Article information

DOI
https://doi.org/10.1039/C2AY25318F
Article type
Communication
Submitted
28 Mar 2012
Accepted
29 Apr 2012
First published
30 Apr 2012

Anal. Methods, 2012,4, 1903-1905

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Bovine serum albumin-coated quantum dots as a cytoplasmic viscosity probe in a single living cell

Y. Nakane, A. Sasaki, M. Kinjo and T. Jin, Anal. Methods, 2012, 4, 1903 DOI: 10.1039/C2AY25318F

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