Issue 6, 2010

A graphene-enhanced molecular beacon for homogeneous DNA detection

Abstract

In this work, we report the design of a novel graphene-based molecular beacon (MB) that could sensitively and selectively detect specific DNA sequences. The ability of water-soluble graphene oxide (GO) to differentiated hairpin and dsDNA offered a new approach to detect DNA. We found that the background fluorescence of MB was significantly suppressed in the presence of GO, which increased the signal-to-background ratio, hence the sensitivity. Moreover, the single-mismatch differentiation ability of hairpin DNA was maintained, leading to high selectivity of this new method.

Graphical abstract: A graphene-enhanced molecular beacon for homogeneous DNA detection

Article information

Article type
Paper
Submitted
10 Dec 2009
Accepted
01 Mar 2010
First published
11 May 2010

Nanoscale, 2010,2, 1021-1026

A graphene-enhanced molecular beacon for homogeneous DNA detection

F. Li, Y. Huang, Q. Yang, Z. Zhong, D. Li, L. Wang, S. Song and C. Fan, Nanoscale, 2010, 2, 1021 DOI: 10.1039/B9NR00401G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements