Issue 6, 2011
From the journal:

Analyst

Electrochemical detection of interaction between Thioflavin T and acetylcholinesterase†

Vladimir Dounin,a   Andrea Constantinof,a   Holger Schulze,b   Till T. Bachmannb  and  Kagan Kerman*a  

* Corresponding authors

a Dept. of Physical and Environmental Sciences, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON, Canada
E-mail: kagan.kerman@utoronto.ca
Tel: +1-416-287-7250

b Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor's Building, 49 Little France Crescent, Edinburgh, UK

Abstract

The electrochemical oxidation of the benzothiazole dye Thioflavin T (ThT) was found to be modulated by its interaction with electric eel acetylcholinesterase (AChE). Modifications of AChE by trace amounts of small molecule inhibitors such as carbachol and paraoxon were detectable electrochemically using minimal reagents and with greater sensitivity than attainable through conventional fluorescence approaches. This property appears to be unique to ThT, since its closely related neutral derivative BTA-1 only interacts with AChE, but is not significantly affected by the presence of small molecule inhibitors.

Graphical abstract: Electrochemical detection of interaction between Thioflavin T and acetylcholinesterase

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Article information

DOI
https://doi.org/10.1039/C0AN00743A
Article type
Paper
Submitted
23 Sep 2010
Accepted
09 Dec 2010
First published
25 Jan 2011

Analyst, 2011,136, 1234-1238

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Electrochemical detection of interaction between Thioflavin T and acetylcholinesterase

V. Dounin, A. Constantinof, H. Schulze, T. T. Bachmann and K. Kerman, Analyst, 2011, 136, 1234 DOI: 10.1039/C0AN00743A

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