Issue 1, 2011
From the journal:

Chemical Communications

Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase†‡

Yana Berezovskaya,a   Craig T. Armstrong,b   Aimee L. Boyle,b   Massimiliano Porrini,a   Derek N. Woolfson*bc  and  Perdita E. Barran*a  

* Corresponding authors

a School of Chemistry, University of Edinburgh, Edinburgh, UK
E-mail: Perdita.Barran@ed.ac.uk
Fax: 13 1650 7533
Tel: 13 1650 7533

b School of Chemistry, University of Bristol, Bristol, UK

c Department of Biochemistry, University of Bristol, Bristol, UK
E-mail: D.N.Woolfson@bristol.ac.uk
Fax: 11 7929 8611
Tel: 11 7954 6347

Abstract

Solution-phase spectroscopy and mass spectrometry are used to probe interactions between divalent metal ions and a synthetic Cys2His2 zinc-finger peptide (vCP1). Both methods provide the same order of binding affinity, zinc ≥ cobalt ≫ copper ≫ calcium. Collision-cross-section measurements show that both apo and holo forms are compact. This is corroborated by molecular-dynamics simulations.

Graphical abstract: Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

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Article information

DOI
https://doi.org/10.1039/C0CC02445G
Article type
Communication
Submitted
19 Jul 2010
Accepted
01 Sep 2010
First published
20 Sep 2010

Chem. Commun., 2011,47, 412-414

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Metal binding to a zinc-finger peptide: a comparison between solution and the gas phase

Y. Berezovskaya, C. T. Armstrong, A. L. Boyle, M. Porrini, D. N. Woolfson and P. E. Barran, Chem. Commun., 2011, 47, 412 DOI: 10.1039/C0CC02445G

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