Issue 13, 2011

Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis

Abstract

Real time PCR is the mainstay of current nucleic acid assays, underpinning applications in forensic science, point-of-care diagnostics and detection of bioterrorism agents. Despite its broad utility, the search for new tests continues, inspired by second and third generation DNA sequencing technologies and fuelled by progress in single molecule fluorescence spectroscopy, nanotechnology and microfabrication. These new methods promise the direct detection of nucleic acids without the need for enzymatic amplification. In this feature article, we provide a chemist's perspective on this multidisciplinary area, introducing the concepts of single molecule detection then focussing on the selection of labels and probe chemistry suitable for generating a signal detectable by ultrasensitive fluorescence spectroscopy. Finally, we discuss the further developments that are required to incorporate these detection platforms into integrated ‘sample-in-answer-out’ instruments, capable of detecting many target sequences in a matter of minutes.

Graphical abstract: Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis

Article information

Article type
Feature Article
Submitted
04 Oct 2010
Accepted
05 Jan 2011
First published
31 Jan 2011

Chem. Commun., 2011,47, 3717-3735

Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis

R. T. Ranasinghe and T. Brown, Chem. Commun., 2011, 47, 3717 DOI: 10.1039/C0CC04215C

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