Issue 9, 2011
From the journal:

Physical Chemistry Chemical Physics

Fluorescence energy transfer efficiency in labeled yeast cytochrome c: a rapid screen for ion biocompatibility in aqueous ionic liquids†

Sheila N. Baker,a   Hua Zhao,b   Siddharth Pandey,c   William T. Heller,a   Frank V. Brightd  and  Gary A. Baker*a  

* Corresponding authors

a Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, USA
E-mail: bakerga1@ornl.gov
Fax: +1 865-576-5235
Tel: +1 865-241-9361

b Chemistry Program, Savannah State University, Savannah, USA

c Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi-110016, India

d Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, USA

Abstract

A fluorescence energy transfer “de-quenching” assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.

Graphical abstract: Fluorescence energy transfer efficiency in labeled yeast cytochrome c: a rapid screen for ion biocompatibility in aqueous ionic liquids

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Article information

DOI
https://doi.org/10.1039/C0CP02345K
Article type
Communication
Submitted
01 Nov 2010
Accepted
14 Dec 2010
First published
13 Jan 2011

Phys. Chem. Chem. Phys., 2011,13, 3642-3644

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Fluorescence energy transfer efficiency in labeled yeast cytochrome c: a rapid screen for ion biocompatibility in aqueous ionic liquids

S. N. Baker, H. Zhao, S. Pandey, W. T. Heller, F. V. Bright and G. A. Baker, Phys. Chem. Chem. Phys., 2011, 13, 3642 DOI: 10.1039/C0CP02345K

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