Issue 5, 2011

GlutathioneS-transferase as a general and reversible tag for surface immobilization of proteins

Abstract

The ability to immobilize biomolecules on nanopatterned substrates is important for diverse applications including tissue engineering and proteomics. The design of general linkers can allow a single substrate to be used for multiple purposes, as well as reconfiguration of devices if the linkage is reversible. In this work, the glutathione S-transferase (GST) tag was used to reversibly immobilize green fluorescent protein (GFP) and basic fibroblast growth factor (FGF2) on glutathione (GSH)-functionalized nanopatterns fabricated by electron-beam lithography.

Graphical abstract: Glutathione S-transferase as a general and reversible tag for surface immobilization of proteins

Supplementary files

Article information

Article type
Paper
Submitted
23 Jul 2010
Accepted
23 Sep 2010
First published
27 Oct 2010

J. Mater. Chem., 2011,21, 1457-1461

Glutathione S-transferase as a general and reversible tag for surface immobilization of proteins

C. M. Kolodziej, C. Chang and H. D. Maynard, J. Mater. Chem., 2011, 21, 1457 DOI: 10.1039/C0JM02370A

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