Issue 10, 2010

Cytotoxic copper(ii) salicylaldehyde semicarbazone complexes: Mode of action and proteomic analysis

Abstract

The in vitro cytotoxic studies of a series of salicylaldehyde semicarbazones, HOC6H4CH[double bond, length as m-dash]N–NHCONR2 (H2R2) and their Cu(II) complexes on a number of human tumor cell lines were conducted and it was observed that their cytotoxicities were enhanced following complexation to copper. These copper(II) complexes also demonstrated higher in vitro activities than the reference drug, cisplatin, on the tumor cell lines at micro molar range. Apoptotic assays and cell cycle analysis of the copper complexes, [Cu(HBnz2)Cl] and [Cu(HBu2)Cl] revealed that they mediated cytotoxicity in MOLT-4 cells via apoptosis. Further proteomic investigation of [Cu(HBnz2)Cl] and [Cu(HBu2)Cl] with respect to their protein expression profiles associated with their mode of action was conducted. By comparing the expression levels of 33 identified protein spots amongst the respective compound-treated profiles, we identified similarities in protein expression patterns between the two copper(II) complexes. The possible roles of the identified proteins in the execution of apoptosis by these copper(II) complexes are discussed.

Graphical abstract: Cytotoxic copper(ii) salicylaldehyde semicarbazone complexes: Mode of action and proteomic analysis

Supplementary files

Article information

Article type
Paper
Submitted
24 May 2010
Accepted
22 Jul 2010
First published
31 Aug 2010

Metallomics, 2010,2, 694-705

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