Issue 20, 2011

Optical trapping for the characterization of amyloid-beta aggregation kinetics

Abstract

Alzheimer's disease (AD) is marked by the accumulation of neuronal plaques from insoluble amyloid-beta (Aβ) peptides. Growing evidence for the role of Aβ oligomers in neuronal cell cytotoxicity and pathogenesis has prompted the development of novel techniques to better understand the early stages of aggregation. Near infrared (NIR) optical trapping was applied to characterize the early stages of Aβ aggregation in the presence of a β-sheet intercalating dye, Congo Red (CR), as the fluorescent marker. The integration of fluorescence analysis with NIR optical trapping has provided a new outlook into the first two hours of Aβ aggregation.

Graphical abstract: Optical trapping for the characterization of amyloid-beta aggregation kinetics

Supplementary files

Article information

Article type
Paper
Submitted
10 Jun 2011
Accepted
02 Aug 2011
First published
26 Aug 2011

Analyst, 2011,136, 4164-4167

Optical trapping for the characterization of amyloid-beta aggregation kinetics

A. J. Veloso, H. Yoshikawa, X. R. Cheng, E. Tamiya and K. Kerman, Analyst, 2011, 136, 4164 DOI: 10.1039/C1AN15480J

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