Direct write tethered protein arrays

Abstract

Existing methods for fabricating protein arrays and patterns on solid surfaces rely upon multiple-step chemical reaction procedures. A more straightforward approach is described where proteins are written directly onto a protein resistant surface which eliminates the need for back-filling. This is achieved by the spatially controlled delivery of protein-containing activation solutions onto poly(N-acryloylsarcosine methyl ester) protein-resistant surfaces. The surface tethered proteins are shown to retain their biological function whilst the surrounding poly(N-acryloylsarcosine methyl ester) background prohibits non-specific protein binding.