Issue 4, 2012

Comparative proteomics to evaluate multi drug resistance in Escherichia coli

Abstract

Drug resistance in food-borne bacterial pathogens is an almost inevitable consequence of the use of antimicrobial drugs, used either therapeutically or to avoid infections in food-producing animals. In the past decades, the spread and inappropriate use of antibiotics have caused a considerable increase of antibiotics to which bacteria have developed resistance and, moreover, bacteria are becoming resistant to more than one antibiotic simultaneously. Understanding mechanisms at the molecular level is extremely important to control multi-resistant strains and to develop new therapeutic strategies. In the present study, comparative proteomics was applied to characterize membrane and cytosolic proteome in order to investigate the regulation of protein expression in multi-resistance E. coli isolated from young never vaccinated water buffalo. Results highlighted differentially expressed proteins under multi drug resistance conditions giving new insights about mechanisms involved in resistance, as quorum sensing mechanisms, and suggesting possible novel bacterial targets to develop alternative antibiotic drugs.

Graphical abstract: Comparative proteomics to evaluate multi drug resistance in Escherichia coli

Article information

Article type
Paper
Submitted
23 Sep 2011
Accepted
08 Nov 2011
First published
28 Nov 2011

Mol. BioSyst., 2012,8, 1060-1067

Comparative proteomics to evaluate multi drug resistance in Escherichia coli

C. Piras, A. Soggiu, L. Bonizzi, A. Gaviraghi, F. Deriu, L. De Martino, G. Iovane, A. Amoresano and P. Roncada, Mol. BioSyst., 2012, 8, 1060 DOI: 10.1039/C1MB05385J

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