Issue 3, 2012

Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium

Abstract

We present a microfluidic epithelial wound-healing assay that allows characterization of the effect of hepatocyte growth factor (HGF) on the regeneration of alveolar epithelium using a flow-focusing technique to create a regular wound in the epithelial monolayer. The phenotype of the epithelial cell was characterized using immunostaining for tight junction (TJ) proteins and transmission electron micrographs (TEMs) of cells cultured in the microfluidic system, a technique that is reported here for the first time. We demonstrate that alveolar epithelial cells cultured in a microfluidic environment preserve their phenotype before and after wounding. In addition, we report a wound-healing benefit induced by addition of HGF to the cell culture medium (19.2 vs. 13.5 μm h−1 healing rate).

Graphical abstract: Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium

Supplementary files

Article information

Article type
Paper
Submitted
13 Sep 2011
Accepted
15 Nov 2011
First published
06 Dec 2011

Lab Chip, 2012,12, 640-646

Microfluidic wound-healing assay to assess the regenerative effect of HGF on wounded alveolar epithelium

M. Felder, P. Sallin, L. Barbe, B. Haenni, A. Gazdhar, T. Geiser and O. Guenat, Lab Chip, 2012, 12, 640 DOI: 10.1039/C1LC20879A

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