Issue 22, 2011

Polymorphism and resolution of oncogene promoter quadruplex-forming sequences

Abstract

We report the separation of several quadruplex species formed by ten promoter sequences by Size Exclusion Chromatography (SEC). Modification at the 5′ or 3′ ends or in loop regions of quadruplex forming sequences has become the standard technique for dealing with quadruplex polymorphism. However, conformations produced employing this method or by other means of artificially shifting the equilibrium may not represent the species that are present in vivo. This method enables an unperturbed view of the structural polymorphism inherent to quadruplex formation. Separation via SEC facilitates studies on quadruplex structure and biophysical properties without the need for sequence modification.

Graphical abstract: Polymorphism and resolution of oncogene promoter quadruplex-forming sequences

Supplementary files

Article information

Article type
Communication
Submitted
03 Jun 2011
Accepted
08 Sep 2011
First published
08 Sep 2011

Org. Biomol. Chem., 2011,9, 7633-7637

Polymorphism and resolution of oncogene promoter quadruplex-forming sequences

M. C. Miller, H. T. Le, W. L. Dean, P. A. Holt, J. B. Chaires and J. O. Trent, Org. Biomol. Chem., 2011, 9, 7633 DOI: 10.1039/C1OB05891F

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