Issue 10, 2012

Rapid analysis of whole blood by paper spray mass spectrometry for point-of-care therapeutic drug monitoring

Abstract

Paper spray mass spectrometry is applied to oncology drugs in fresh whole blood samples supported on filter paper substrates instead of dry blood as done previously. Addition of the coagulant alum clotted the blood and allowed for immediate sample analysis. The coagulant did not interfere with the function of the paper spray nor did it add features to the mass spectra. Quantitative analysis of therapeutic drugs in the blood was achieved utilizing internal standards which were pre-spotted onto the filter paper. Eight oncology drugs were examined, with lower limits of detection ranging between 0.5 and 17 ng mL−1 and linear dynamic ranges greater than two orders of magnitude. Inter-day accuracies of quality controls for pazopanib ranged from 102 to 118%, with imprecisions of 9 to 13%. This one-step method requires 10 μL of blood, a drop of solvent, and takes 45 seconds per trial. These results indicate applicability to point-of-care therapeutic drug monitoring in a clinical setting.

Graphical abstract: Rapid analysis of whole blood by paper spray mass spectrometry for point-of-care therapeutic drug monitoring

Article information

Article type
Paper
Submitted
16 Jan 2012
Accepted
18 Mar 2012
First published
20 Mar 2012

Analyst, 2012,137, 2344-2349

Rapid analysis of whole blood by paper spray mass spectrometry for point-of-care therapeutic drug monitoring

R. D. Espy, N. E. Manicke, Z. Ouyang and R. G. Cooks, Analyst, 2012, 137, 2344 DOI: 10.1039/C2AN35082C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements