A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles

Hongjun Lin; Jingrui Huo; Aihong Zhang; Yingfu Liu; Qingming Wang; Yun Cai; Wantao Ying; Weijie Qin; Yangjun Zhang; Xiaohong Qian

doi:10.1039/C2AN35242G

A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles

Hongjun Lin,^a Jingrui Huo,^a Aihong Zhang,^b Yingfu Liu,^a Qingming Wang,^a Yun Cai,^a Wantao Ying,^a Weijie Qin,^a Yangjun Zhang*^a and Xiaohong Qian*^a

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^a State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China
E-mail: qianxh1@163.com, zhangyangjun6314@yahoo.com.cn
Tel: +86-10-80727777-1146/1231

^b Institute of Chemical Defense, Beijing 102205, China

Abstract

In this study, we report an ultrasensitive western blotting method using antibody-functionalised graphene oxide sheets and gold nanoparticles. Additionally, the cost is reduced greatly by conjugating two different primary antibodies on gold nanoparticles (C. Welinder and L. Ekblad, J. Proteome Res., 2011, 10, 1416–1419).

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A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles

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A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles

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