Issue 17, 2012

13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall

Abstract

The ability to accurately and rapidly measure plant cell wall composition, relative monolignol content and lignin–hemicellulose inter-unit linkage distributions has become essential to efforts centered on reducing the recalcitrance of biomass by genetic engineering. Growing 13C enriched transgenic plants is a viable route to achieve the high-throughput, detailed chemical analysis of whole plant cell wall before and after pretreatment and microbial or enzymatic utilization by 13C nuclear magnetic resonance (NMR) in a perdeuterated ionic liquid solvent system not requiring component isolation. 1D 13C whole cell wall ionic liquid NMR of natural abundant and 13C enriched corn stover stem samples suggest that a high level of uniform labeling (>97%) can significantly reduce the total NMR experiment times up to ∼220 times. Similarly, significant reduction in total NMR experiment time (∼39 times) of the 13C enriched corn stover stem samples for 2D 13C–1H heteronuclear single quantum coherence NMR was found.

Graphical abstract: 13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall

Article information

Article type
Communication
Submitted
13 Mar 2012
Accepted
29 May 2012
First published
05 Jul 2012

Analyst, 2012,137, 3904-3909

13C cell wall enrichment and ionic liquid NMR analysis: progress towards a high-throughput detailed chemical analysis of the whole plant cell wall

M. Foston, R. Samuel and A. J. Ragauskas, Analyst, 2012, 137, 3904 DOI: 10.1039/C2AN35344J

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