Issue 4, 2012

Quality assurance in immunoassay performance-temperature effects

Abstract

Temperature is one factor that influences the performance of immunoassays. Most commonly all incubation steps of the immunoassay are performed at ambient temperature. To systematically study the influence of temperature deviations on assay parameters like signal variation coefficients, limit of detection and measurement range, direct competitive enzyme immunoassays for the determination of two anthropogenic markers, caffeine and carbamazepine, were run at different temperatures above and below room temperature during individual assay steps. The temperature during the competition step had the greatest impact on the assay sensitivity yielding changes in test midpoints by a factor of 10 to 15. For carbamazepine, the test midpoints varied from 36 ng L−1, 108 ng L−1 to 378 ng L−1 employing 4 °C, 21 °C, or 37 °C as incubation temperature, respectively. The edge effect can be minimized resulting in a good plate homogeneity with small variation coefficients, when the assay is performed at ambient temperature.

Graphical abstract: Quality assurance in immunoassay performance-temperature effects

Article information

Article type
Paper
Submitted
20 Dec 2011
Accepted
07 Feb 2012
First published
05 Mar 2012

Anal. Methods, 2012,4, 901-905

Quality assurance in immunoassay performance-temperature effects

J. Grandke, U. Resch-Genger, W. Bremser, L. Garbe and R. J. Schneider, Anal. Methods, 2012, 4, 901 DOI: 10.1039/C2AY05918E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements