Issue 6, 2012

Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry

Abstract

Because of its rich π electrons, graphene oxide (GO) can bind with single-stranded DNA (ssDNA) through π-π stacking, but it cannot bind with double-stranded DNA (dsDNA). This different binding ability of GO with DNA has found many applications. By combining this ability with aptamer chemistry, we have developed a highly selective method for the detection of adenosine triphosphate (ATP). The hybrid of ATP aptamer with fluorescein (FAM)-labelled complementary DNA (FAM-DNA) demonstrates a weak affinity for GO and strong fluorescence of FAM-DNA. If ATP is present the strong fluorescence of FAM-DNA is quenched, because the binding of ATP with its aptamer greatly inhibits hybridization of the aptamer with FAM-DNA, and the unhybridized FAM-DNA is adsorbed on to the surface of GO. The quenched fluorescence intensity was found to be in proportion to the concentration of ATP in the range 3–320 μM with a detection limit of 0.45 μM. This method of ATP detection is highly selective and the existence of GTP, CTP and UTP have scarcely effect the determination.

Graphical abstract: Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry

Supplementary files

Article information

Article type
Paper
Submitted
16 Feb 2012
Accepted
27 Mar 2012
First published
28 Mar 2012

Anal. Methods, 2012,4, 1662-1666

Graphene oxide as a nano-platform for ATP detection based on aptamer chemistry

W. D. Pu, L. Zhang and C. Z. Huang, Anal. Methods, 2012, 4, 1662 DOI: 10.1039/C2AY25166C

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