Issue 9, 2012
From the journal:

Analytical Methods

Hierarchical Fe-ZSM-5 zeolite monolithic column for enhanced phosphorylated protein immobilization and identification†

Wenzhong Zhang,‡a   Deju Wang,‡b   Huai Sun,c   Jun Yao,d   Fang Xu*a  and  Pengyuan Yangd  

* Corresponding authors

a School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
E-mail: xufangzh@sjtu.edu.cn
Fax: +86 21 54741065
Tel: +86 21 34201548

b Shanghai Research Institute of Petrochemical Technology, Sinopec, Shanghai 201208, China

c School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, Shanghai 200240, China

d Institutes of Biomedical Sciences, Department of Chemistry, Fudan University, Shanghai 200443, China

Abstract

A hierarchical Fe-ZSM-5 zeolite monolithic column was fabricated for immobilization of low abundance phosphoprotein through specific coordination between heteroatomic Fe in the framework of the zeolite with the phosphate group in the micro-environment of the protein. Compared to traditionally immobilized metal ion chromatography, this method could be directly manipulated and handily adapted for phosphoproteomics identification.

Graphical abstract: Hierarchical Fe-ZSM-5 zeolite monolithic column for enhanced phosphorylated protein immobilization and identification

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Article information

DOI
https://doi.org/10.1039/C2AY25454A
Article type
Communication
Submitted
02 Apr 2012
Accepted
07 Jul 2012
First published
09 Jul 2012

Anal. Methods, 2012,4, 2644-2647

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Hierarchical Fe-ZSM-5 zeolite monolithic column for enhanced phosphorylated protein immobilization and identification

W. Zhang, D. Wang, H. Sun, J. Yao, F. Xu and P. Yang, Anal. Methods, 2012, 4, 2644 DOI: 10.1039/C2AY25454A

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