Issue 12, 2012

Preparation of aligned porous silica monolithic capillary columns and their evaluation for HPLC

Abstract

Porous monolithic columns have been investigated and used to achieve fast separation with low back pressure and good resolution. Silica monoliths are favoured over polymeric monoliths due to their chemical/mechanical stability and non-swelling property. The monolithic columns with randomly distributed pore structures have been used so far, leading to eddy diffusion and peak broadening. Here, a directional freezing and freeze drying method is used for the first time to produce aligned porous silica monoliths in a silica capillary from commercially available silica colloidal suspension. Both silica and silicapolymer composite monoliths are produced and the full pore characterizations are performed. These monoliths contain parallel microchannels and thus minimize eddy diffusion. The HPLC tests on these monoliths under both normal phase and reversed phase conditions demonstrate the feasibility for use in chromatography. The aligned microchannel structures of the monoliths can achieve efficient separation with significantly low back pressure, compared to conventional porous monoliths. There is great potential to further develop this new type of monoliths for highly efficient HPLC with low operating back pressure.

Graphical abstract: Preparation of aligned porous silica monolithic capillary columns and their evaluation for HPLC

Article information

Article type
Paper
Submitted
28 Jun 2012
Accepted
28 Sep 2012
First published
01 Oct 2012

Anal. Methods, 2012,4, 3942-3947

Preparation of aligned porous silica monolithic capillary columns and their evaluation for HPLC

A. Ahmed, P. Myers and H. Zhang, Anal. Methods, 2012, 4, 3942 DOI: 10.1039/C2AY25671A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements