Issue 10, 2012

A nanocomposite based biosensor for cholesterol determination

Abstract

An electrochemical biosensor was prepared by immobilizing cholesterol oxidase (ChOx) on a nanocomposite consisting of amine functionalized multi-walled carbon nanotubes and a room temperature ionic-liquid (1-butyl-3-methylimidazolium tetrafluoroborate). The biosensor was examined for cholesterol quantification in clinical serum samples. ChOx on the modified electrode exhibited a couple of quasi-reversible redox peaks corresponding to the direct electron transfer of FAD/FADH2 buried in the enzyme structure. A formal potential of −400 mV was obtained for the immobilized enzyme in phosphate buffer solution (0.1 M, pH 7.4). Under the optimized experimental conditions, the biosensor exhibited a linear response towards cholesterol concentration from 26 nM to 3.4 μM, with a sensitivity of 540 μA mM−1and a detection limit of 12 nM.

Graphical abstract: A nanocomposite based biosensor for cholesterol determination

Article information

Article type
Paper
Submitted
28 Mar 2012
Accepted
12 Aug 2012
First published
13 Aug 2012

Anal. Methods, 2012,4, 3225-3231

A nanocomposite based biosensor for cholesterol determination

S. Karimi, H. Ghourchian, P. Rahimi and H. Rafiee-Pour, Anal. Methods, 2012, 4, 3225 DOI: 10.1039/C2AY25826A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements