Issue 4, 2012

Proteome profile and biological activity of caprine, bovine and human milk fat globules

Abstract

Upon combining bidimensional electrophoresis with monodimensional separation, a more comprehensive analysis of the milk fat globule membrane has been obtained. The proteomic profile of caprine milk fat globules revealed the presence of butyrophilin, lactadherin and perilipin as the major proteins, they were also associated to bovine and human milk fat globule membranes. Xanthine dehydrogenase/oxidase has been detected only in monodimensional gels. Biological activity of milk fat globules has been evaluated in Caco2-cells, as a representative model of the intestinal barrier. The increase of cell viability was indicative of a potential nutraceutical role for the whole milk fat globule, suggesting a possible employment in milk formula preparation.

Graphical abstract: Proteome profile and biological activity of caprine, bovine and human milk fat globules

Article information

Article type
Paper
Submitted
27 Sep 2011
Accepted
02 Dec 2011
First published
21 Dec 2011

Mol. BioSyst., 2012,8, 967-974

Proteome profile and biological activity of caprine, bovine and human milk fat globules

S. Spertino, V. Cipriani, C. De Angelis, M. G. Giuffrida, F. Marsano and M. Cavaletto, Mol. BioSyst., 2012, 8, 967 DOI: 10.1039/C2MB05400K

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