Issue 10, 2012

Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range

Abstract

Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.

Graphical abstract: Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range

Supplementary files

Article information

Article type
Communication
Submitted
05 Apr 2012
Accepted
08 Jun 2012
First published
13 Jun 2012

Mol. BioSyst., 2012,8, 2531-2534

Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range

S. Milles, C. Koehler, Y. Gambin, A. A. Deniz and E. A. Lemke, Mol. BioSyst., 2012, 8, 2531 DOI: 10.1039/C2MB25135C

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