Issue 23, 2012
From the journal:

Organic & Biomolecular Chemistry

Strategy for catch and release of azide-tagged biomolecules utilizing a photolabile strained alkyne construct†

Martin Golkowski,a   Carlo Pergola,b   Oliver Werzb  and  Thomas Ziegler*a  

* Corresponding authors

a Institute of Organic Chemistry, University of Tübingen, Auf der Morgenstelle 18, 72076 Tübingen, Germany
E-mail: Thomas.Ziegler@uni-tuebingen.de
Fax: +49 (0)7071 29-5244
Tel: +49 (0)7071 29-78763

b Institute of Pharmaceutical/Medicinal Chemistry, University of Jena, Philosophenweg 14, 07743 Jena, Germany
E-mail: Oliver.Werz@uni-jena.de
Fax: +49 (0)3641-949802
Tel: +49 (0)3641-949801

Abstract

A photolabile o-nitrobenzyl linker–cyclooctyne conjugate was prepared, immobilized on poly(methacrylate) beads and utilized as a trap for azide-functionalized compounds. These could be released by 365 nm UV light irradiation in high yield and purity. The “reagent-free” and time economic catch and release protocol was deemed useful for chemical proteomics applications.

Graphical abstract: Strategy for catch and release of azide-tagged biomolecules utilizing a photolabile strained alkyne construct

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Article information

DOI
https://doi.org/10.1039/C2OB25440A
Article type
Communication
Submitted
29 Feb 2012
Accepted
26 Apr 2012
First published
26 Apr 2012

Org. Biomol. Chem., 2012,10, 4496-4499

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Strategy for catch and release of azide-tagged biomolecules utilizing a photolabile strained alkyne construct

M. Golkowski, C. Pergola, O. Werz and T. Ziegler, Org. Biomol. Chem., 2012, 10, 4496 DOI: 10.1039/C2OB25440A

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