Issue 2, 2013

A photochromic bacterial photoreceptor with potential for super-resolution microscopy

Abstract

We introduce a novel fluorescent reporter with potential for super-resolution microscopy, based on the bacterial photoreceptor YtvA. YtvA (from Bacillus subtilis) comprises a photosensitive flavin-based LOV domain, efficiently photo-switchable between fluorescent and non-fluorescent states. We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules.

Graphical abstract: A photochromic bacterial photoreceptor with potential for super-resolution microscopy

Supplementary files

Article information

Article type
Communication
Submitted
13 Jul 2012
Accepted
20 Sep 2012
First published
21 Sep 2012

Photochem. Photobiol. Sci., 2013,12, 231-235

A photochromic bacterial photoreceptor with potential for super-resolution microscopy

A. Losi, W. Gärtner, S. Raffelberg, F. Cella Zanacchi, P. Bianchini, A. Diaspro, C. Mandalari, S. Abbruzzetti and C. Viappiani, Photochem. Photobiol. Sci., 2013, 12, 231 DOI: 10.1039/C2PP25254F

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