Issue 17, 2013

Multiplexed detection of microRNAs by tuning DNA-scaffolded silver nanoclusters

Abstract

A universal silver-nanocluster method coupled with target-triggered isothermal exponential amplification reaction (TIEAR) is developed for light-up fluorescent detection of multiple microRNAs (miRNAs) in a label-free format. Taking advantage of the interesting feature of the fine-tuned emission spectrum of fluorescent DNA-scaffolded silver nanoclusters (DNA/AgNCs), our proposed assay is designed such that the different miRNA targets are transferred to the different oligonucleotide reporters via the TIEAR, in which unimolecular DNAs designed for different targets are employed as the amplification templates, polymerases and nicking enzymes as mechanical activators and miRNA targets as the trigger. The produced oligonucleotide reporters act as templates for the synthesis of multicolor DNA/AgNC probes, which correspond to different target inputs. This proposed method has been well validated on the multiplex detection of miRNAs and DNAs, as well as in practical application.

Graphical abstract: Multiplexed detection of microRNAs by tuning DNA-scaffolded silver nanoclusters

Supplementary files

Article information

Article type
Paper
Submitted
03 Apr 2013
Accepted
04 Jun 2013
First published
04 Jun 2013

Analyst, 2013,138, 4812-4817

Multiplexed detection of microRNAs by tuning DNA-scaffolded silver nanoclusters

M. Zhang, Y. Liu, C. Yu, B. Yin and B. Ye, Analyst, 2013, 138, 4812 DOI: 10.1039/C3AN00666B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements