Issue 20, 2013
From the journal:

Analyst

Convenient detection of E. coli in Ringer's solution

Martha Schwarz,†a   Susanne Pahlow,†b   Thomas Bocklitz,b   Carolin Steinbrücker,a   Dana Cialla,ab   Karina Weberab  and  Jürgen Popp*ab  

* Corresponding authors

a Institute of Photonic Technology Jena, Albert-Einstein-Straße 9, 07745 Jena, Germany
E-mail: juergen.popp@ipht-jena.de
Fax: +49 3641 206399
Tel: +49 3641 206300

b Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743 Jena, Germany
E-mail: juergen.popp@uni-jena.de
Fax: +49 3641 948302
Tel: +49 3641 948320

Abstract

An easy and inexpensive detection method for DNA hybridization assays combining magnetic beads and enzymatically generated silver nanoparticles is introduced. The main advantage of this approach is the possibility to distinguish between positive and negative test results with the naked eye. In the case of complementary DNA sequences the sample will turn black within a few minutes, allowing readout without any hardware. In order to illustrate the applicability of the assay genomic DNA isolated from E. coli contaminated Ringer's solution was used for testing the sensitivity as well as specificity.

Graphical abstract: Convenient detection of E. coli in Ringer's solution

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Article information

DOI
https://doi.org/10.1039/C3AN01240A
Article type
Communication
Submitted
25 Jun 2013
Accepted
10 Aug 2013
First published
12 Aug 2013

Analyst, 2013,138, 5866-5870

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Convenient detection of E. coli in Ringer's solution

M. Schwarz, S. Pahlow, T. Bocklitz, C. Steinbrücker, D. Cialla, K. Weber and J. Popp, Analyst, 2013, 138, 5866 DOI: 10.1039/C3AN01240A

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