Issue 3, 2014
From the journal:

Analyst

Real-time evaluation of aggregation using confocal imaging and image analysis tools†

Zahra Hamrang,*a   Egor Zindy,ab   David Clarkeac  and  Alain Pluen*ac  

* Corresponding authors

a School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester, UK
E-mail: zahra.hamrang@manchester.ac.uk, alain.pluen@manchester.ac.uk

b The Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, UK

c Centre of Excellence in Biopharmaceuticals, University of Manchester, Manchester, UK

Abstract

Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.

Graphical abstract: Real-time evaluation of aggregation using confocal imaging and image analysis tools

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Article information

DOI
https://doi.org/10.1039/C3AN01693E
Article type
Communication
Submitted
06 Sep 2013
Accepted
20 Nov 2013
First published
20 Nov 2013

Analyst, 2014,139, 564-568

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Real-time evaluation of aggregation using confocal imaging and image analysis tools

Z. Hamrang, E. Zindy, D. Clarke and A. Pluen, Analyst, 2014, 139, 564 DOI: 10.1039/C3AN01693E

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