Issue 5, 2014

Human versus porcine tissue sourcing for an injectable myocardial matrix hydrogel

Abstract

Heart failure (HF) after myocardial infarction (MI) is a leading cause of death in the western world with a critical need for new therapies. A previously developed injectable hydrogel derived from porcine myocardial matrix (PMM) has had successful results in both small and large animal MI models. In this study, we sought to evaluate the impact of tissue source on this biomaterial, specifically comparing porcine and human myocardium sources. We first developed an analogous hydrogel derived from human myocardial matrix (HMM). The biochemical and physical properties of the PMM and HMM hydrogels were then characterized, including residual dsDNA, protein content, sulfated glycosaminoglycan (sGAG) content, complex viscosity, storage and loss moduli, and nano-scale topography. Biochemical activity was investigated with in vitro studies for the proliferation of vascular cells and differentiation of human cardiomyocyte progenitor cells (hCMPCs). Next, in vivo gelation and material spread were confirmed for both PMM and HMM after intramyocardial injection. After extensive comparison, the matrices were found to be similar, yet did show some differences. Because of the rarity of collecting healthy human hearts, the increased difficulty in processing the human tissue, shifts in extracellular matrix (ECM) composition due to aging, and significant patient-to-patient variability, these studies suggest that the HMM is not a viable option as a scalable product for the clinic; however, the HMM has potential as a tool for in vitro cell culture.

Graphical abstract: Human versus porcine tissue sourcing for an injectable myocardial matrix hydrogel

Article information

Article type
Paper
Submitted
12 Nov 2013
Accepted
15 Jan 2014
First published
21 Jan 2014

Biomater. Sci., 2014,2, 735-744

Author version available

Human versus porcine tissue sourcing for an injectable myocardial matrix hydrogel

T. D. Johnson, J. A. DeQuach, R. Gaetani, J. Ungerleider, D. Elhag, V. Nigam, A. Behfar and K. L. Christman, Biomater. Sci., 2014, 2, 735 DOI: 10.1039/C3BM60283D

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