Issue 24, 2013

In-chip fabrication of free-form 3D constructs for directed cell migration analysis

Abstract

Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.

Graphical abstract: In-chip fabrication of free-form 3D constructs for directed cell migration analysis

Supplementary files

Article information

Article type
Paper
Submitted
09 Aug 2013
Accepted
27 Sep 2013
First published
30 Sep 2013
This article is Open Access
Creative Commons BY license

Lab Chip, 2013,13, 4800-4809

In-chip fabrication of free-form 3D constructs for directed cell migration analysis

M. H. Olsen, G. M. Hjortø, M. Hansen, Ö. Met, I. M. Svane and N. B. Larsen, Lab Chip, 2013, 13, 4800 DOI: 10.1039/C3LC50930C

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