Issue 44, 2013
From the journal:

Soft Matter

Self-organization of the bacterial cell-division protein FtsZ in confined environments†

Sonia Mellouli,‡a   Begoña Monterroso,‡b   Hanumantha Rao Vutukuri,a   Esra te Brinke,a   Venkatachalam Chokkalingam,a   Germán Rivas§*b  and  Wilhelm T. S. Huck§*a  

* Corresponding authors

a Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands

b Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain

Abstract

We report a microfluidic approach to generate aqueous droplets in oil of different dimensionality, stabilized by a lipid monolayer, to systematically probe the polymerization of bacterial cell-division protein FtsZ into fibrous networks as a function of the concentrations of crowding agent, FtsZ, and GTP. FtsZ bundles confined in droplets were dynamic, and their distribution depended on the intrinsic properties of the system and restrictions imposed by the spatial boundaries.

Graphical abstract: Self-organization of the bacterial cell-division protein FtsZ in confined environments

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Article information

DOI
https://doi.org/10.1039/C3SM51163D
Article type
Paper
Submitted
26 Apr 2013
Accepted
27 Jun 2013
First published
27 Jun 2013

Soft Matter, 2013,9, 10493-10500

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Self-organization of the bacterial cell-division protein FtsZ in confined environments

S. Mellouli, B. Monterroso, H. R. Vutukuri, E. T. Brinke, V. Chokkalingam, G. Rivas and W. T. S. Huck, Soft Matter, 2013, 9, 10493 DOI: 10.1039/C3SM51163D

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