Issue 15, 2014

Testing Aβ toxicity on primary CNS cultures using drug-screening microfluidic chips

Abstract

Open microscale cultures of primary central nervous system (CNS) cells have been implemented in microfluidic chips that can expose the cells to physiological fluidic shear stress conditions. Cells in the chips were exposed to differently aggregated forms of beta-amyloid (Aβ), i.e. conditions mimicking an Alzheimer's Disease environment, and treated with CNS drugs in order to assess the contribution of glial cells during pharmacological treatments. FTY720, a drug approved for the treatment of Multiple Sclerosis, was found to play a marked neuroprotective role in neuronal cultures as well as in microglia-enriched neuronal cultures, preventing neurodegeneration after cell exposure to neurotoxic oligomers of Aβ.

Graphical abstract: Testing Aβ toxicity on primary CNS cultures using drug-screening microfluidic chips

Supplementary files

Article information

Article type
Paper
Submitted
10 Feb 2014
Accepted
17 May 2014
First published
19 May 2014

Lab Chip, 2014,14, 2860-2866

Author version available

Testing Aβ toxicity on primary CNS cultures using drug-screening microfluidic chips

A. Ruiz, P. Joshi, R. Mastrangelo, M. Francolini, C. Verderio and M. Matteoli, Lab Chip, 2014, 14, 2860 DOI: 10.1039/C4LC00174E

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