Issue 17, 2014

Microfluidics for single-cell genetic analysis

Abstract

The ability to correlate single-cell genetic information to cellular phenotypes will provide the kind of detailed insight into human physiology and disease pathways that is not possible to infer from bulk cell analysis. Microfluidic technologies are attractive for single-cell manipulation due to precise handling and low risk of contamination. Additionally, microfluidic single-cell techniques can allow for high-throughput and detailed genetic analyses that increase accuracy and decrease reagent cost compared to bulk techniques. Incorporating these microfluidic platforms into research and clinical laboratory workflows can fill an unmet need in biology, delivering the highly accurate, highly informative data necessary to develop new therapies and monitor patient outcomes. In this perspective, we describe the current and potential future uses of microfluidics at all stages of single-cell genetic analysis, including cell enrichment and capture, single-cell compartmentalization and manipulation, and detection and analyses.

Graphical abstract: Microfluidics for single-cell genetic analysis

Article information

Article type
Frontier
Submitted
10 Feb 2014
Accepted
31 Mar 2014
First published
31 Mar 2014

Lab Chip, 2014,14, 3135-3142

Author version available

Microfluidics for single-cell genetic analysis

A. M. Thompson, A. L. Paguirigan, J. E. Kreutz, J. P. Radich and D. T. Chiu, Lab Chip, 2014, 14, 3135 DOI: 10.1039/C4LC00175C

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