Issue 6, 2015

Optofluidic ultrahigh-throughput detection of fluorescent drops

Abstract

This paper describes an optofluidic droplet interrogation device capable of counting fluorescent drops at a throughput of 254 000 drops per second. To our knowledge, this rate is the highest interrogation rate published thus far. Our device consists of 16 parallel microfluidic channels bonded directly to a filter-coated two-dimensional Complementary Metal-Oxide-Semiconductor (CMOS) sensor array. Fluorescence signals emitted from the drops are collected by the sensor that forms the bottom of the channel. The proximity of the drops to the sensor facilitates efficient collection of fluorescence emission from the drops, and overcomes the trade-off between light collection efficiency and field of view in conventional microscopy. The interrogation rate of our device is currently limited by the acquisition speed of CMOS sensor, and is expected to increase further as high-speed sensors become increasingly available.

Graphical abstract: Optofluidic ultrahigh-throughput detection of fluorescent drops

Supplementary files

Article information

Article type
Paper
Submitted
15 Dec 2014
Accepted
08 Jan 2015
First published
08 Jan 2015

Lab Chip, 2015,15, 1417-1423

Author version available

Optofluidic ultrahigh-throughput detection of fluorescent drops

M. Kim, M. Pan, Y. Gai, S. Pang, C. Han, C. Yang and S. K. Y. Tang, Lab Chip, 2015, 15, 1417 DOI: 10.1039/C4LC01465K

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