Bimetallic Au/Ag nanoparticle loading on PNIPAAm–VAA–CS8 thermoresponsive hydrogel surfaces using ss-DNA coupling, and their SERS efficiency

Abstract

Thermoresponsive hydrogels can be efficiently used as templates for bimetallic noble metal surface loading for the fabrication of plasmonic surfaces with a wide range of applications. Here, we report, for the first time, an easy approach for bimetallic Au/Ag surface loading by modifying poly(N-isopropylacrylamide) (PNIPAAm) hydrogel surfaces with ss-DNA. The advantages of this approach consist of the accuracy and the simplicity by which both gold and silver nanoparticles can be adsorbed by electrostatic interactions on hydrogel templates, without requiring sophisticated chemical treatment for their conjugation or the growth of nanoparticles on a hydrogel surface. The resulting patterns possess the capability of tuning the interparticle distance upon temperature changes, and thus their plasmonic properties. The aforementioned templates have been successfully used as SERS substrates for 5 × 10⁻⁷ M adenine detection.