Issue 3, 2016

Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell

Abstract

Ion mobility mass spectrometry experiments enable the characterization of mass, assembly, and shape of biological molecules and assemblies. Here, a new radio-frequency confining drift cell is characterized and used to measure the mobilities of peptide, protein, and protein complex ions. The new drift cell replaced the traveling-wave ion mobility cell in a Waters Synapt G2 HDMS. Methods for operating the drift cell and determining collision cross section values using this experimental set up are presented within the context of the original instrument control software. Collision cross sections for 349 cations and anions are reported, 155 of which are for ions that have not been characterized previously using ion mobility. The values for the remaining ions are similar to those determined using a previous radio-frequency confining drift cell and drift tubes without radial confinement. Using this device under 2 Torr of helium gas and an optimized drift voltage, denatured and native-like ions exhibited average apparent resolving powers of 14.2 and 16.5, respectively. For ions with high mobility, which are also low in mass, the apparent resolving power is limited by contributions from ion gating. In contrast, the arrival-time distributions of low-mobility, native-like ions are not well explained using only contributions from ion gating and diffusion. For those species, the widths of arrival-time distributions are most consistent with the presence of multiple structures in the gas phase.

Graphical abstract: Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell

Supplementary files

Article information

Article type
Paper
Submitted
13 Oct 2015
Accepted
23 Dec 2015
First published
04 Jan 2016
This article is Open Access
Creative Commons BY license

Analyst, 2016,141, 884-891

Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell

S. J. Allen, K. Giles, T. Gilbert and M. F. Bush, Analyst, 2016, 141, 884 DOI: 10.1039/C5AN02107C

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements