An acoustofluidic sputum liquefier

Po-Hsun Huang; Liqiang Ren; Nitesh Nama; Sixing Li; Peng Li; Xianglan Yao; Rosemarie A. Cuento; Cheng-Hsin Wei; Yuchao Chen; Yuliang Xie; Ahmad Ahsan Nawaz; Yael G. Alevy; Michael J. Holtzman; J. Philip McCoy; Stewart J. Levine; Tony Jun Huang

doi:10.1039/C5LC00539F

An acoustofluidic sputum liquefier†

Po-Hsun Huang,^a Liqiang Ren,^a Nitesh Nama,^a Sixing Li,^ab Peng Li,^a Xianglan Yao,^c Rosemarie A. Cuento,^c Cheng-Hsin Wei,^d Yuchao Chen,^a Yuliang Xie,^ae Ahmad Ahsan Nawaz,^a Yael G. Alevy,^f Michael J. Holtzman,^f J. Philip McCoy,^c Stewart J. Levine^c and Tony Jun Huang*^ae

Author affiliations

* Corresponding authors

^a Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA
E-mail: junhuang@psu.edu

^b Cell and Developmental Biology (CDB) Graduate Program, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA

^c National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA

^d Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA 16802, USA

^e Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA

^f Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA

Abstract

We demonstrate the first microfluidic-based on-chip liquefaction device for human sputum samples. Our device is based on an acoustofluidic micromixer using oscillating sharp edges. This acoustofluidic sputum liquefier can effectively and uniformly liquefy sputum samples at a throughput of 30 μL min⁻¹. Cell viability and integrity are maintained during the sputum liquefaction process. Our acoustofluidic sputum liquefier can be conveniently integrated with other microfluidic units to enable automated on-chip sputum processing and analysis.

Lab on a Chip

An acoustofluidic sputum liquefier†

Abstract

Supplementary files

Article information

Download Citation

Permissions

An acoustofluidic sputum liquefier

Social activity

Search articles by author

Spotlight

Advertisements