Issue 24, 2016

Single-cell isolation by a modular single-cell pipette for RNA-sequencing

Abstract

Single-cell transcriptome sequencing highly requires a convenient and reliable method to rapidly isolate a live cell into a specific container such as a PCR tube. Here, we report a modular single-cell pipette (mSCP) consisting of three modular components, a SCP-Tip, an air-displacement pipette (ADP), and ADP-Tips, that can be easily assembled, disassembled, and reassembled. By assembling the SCP-Tip containing a hydrodynamic trap, the mSCP can isolate single cells from 5–10 cells per μL of cell suspension. The mSCP is compatible with microscopic identification of captured single cells to finally achieve 100% single-cell isolation efficiency. The isolated live single cells are in submicroliter volumes and well suitable for single-cell PCR analysis and RNA-sequencing. The mSCP possesses merits of convenience, rapidness, and high efficiency, making it a powerful tool to isolate single cells for transcriptome analysis.

Graphical abstract: Single-cell isolation by a modular single-cell pipette for RNA-sequencing

Supplementary files

Article information

Article type
Paper
Submitted
04 Oct 2016
Accepted
02 Nov 2016
First published
02 Nov 2016

Lab Chip, 2016,16, 4742-4748

Single-cell isolation by a modular single-cell pipette for RNA-sequencing

K. Zhang, M. Gao, Z. Chong, Y. Li, X. Han, R. Chen and L. Qin, Lab Chip, 2016, 16, 4742 DOI: 10.1039/C6LC01241H

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