Issue 4, 2010

Click chemistry with DNA

Abstract

The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. The copper catalysed alkyneazide cycloaddition (CuAAC) reaction is the method of choice for DNA click chemistry due to its remarkable efficiency. It has been used to label oligonucleotides with fluorescent dyes, sugars, peptides and other reporter groups, to cyclise DNA, to synthesise DNA catenanes, to join oligonucleotides to PNA, and to produce analogues of DNA with modified nucleobases and backbones. In this critical review we describe some of the pioneering work that has been carried out in this area (78 references).

Graphical abstract: Click chemistry with DNA

Article information

Article type
Critical Review
Submitted
01 Sep 2009
First published
09 Feb 2010

Chem. Soc. Rev., 2010,39, 1388-1405

Click chemistry with DNA

A. H. El-Sagheer and T. Brown, Chem. Soc. Rev., 2010, 39, 1388 DOI: 10.1039/B901971P

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