Issue 12, 2009

Non-covalent biofunctionalization of single-walled carbon nanotubesviabiotin attachment by π-stacking interactions and pyrrolepolymerization

Abstract

Single-walled carbon nanotubes were functionalized with biotin using either electropolymerization or formation of π-stacking interactions for the construction of biosensors. Thanks to the high affinity of the avidin–biotin interactions, a biotinylated glucose oxidase (B-GOX) as a biomolecule model was immobilized on the biotinylated nanotubes. The influence of the biosensor configuration on their amperometric performances was investigated by changing the amount of nanotubes and the numbers of avidin/B-GOX layers. By increasing the amount of nanotube and avidin/B-GOX layers, both sensor setups show a perfect linear increase of immobilized enzymes reflecting a high reproducibility of our systems. The highest sensitivities (up to 5.2 mA M−1 cm−2) and maximum current densities (up to 55 µA cm−2) were obtained using nanotube deposits modified by electrochemical coatings. In contrast, non-covalently functionalized biotin-nanotubes show a better permeability for the enzymatically generated hydrogen peroxide.

Graphical abstract: Non-covalent biofunctionalization of single-walled carbon nanotubesviabiotin attachment by π-stacking interactions and pyrrole polymerization

Article information

Article type
Paper
Submitted
13 Aug 2009
Accepted
22 Sep 2009
First published
29 Sep 2009

Analyst, 2009,134, 2412-2418

Non-covalent biofunctionalization of single-walled carbon nanotubesviabiotin attachment by π-stacking interactions and pyrrole polymerization

R. Haddad, S. Cosnier, A. Maaref and M. Holzinger, Analyst, 2009, 134, 2412 DOI: 10.1039/B916774A

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