Issue 4, 2012
From the journal:

Chemical Communications

Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and Staudinger reactions†

Harita Rao,a   Anupam A. Sawant,a   Arun A. Tanpurea  and  Seergazhi G. Srivatsan*a  

* Corresponding authors

a Indian Institute of Science Education and Research, Sai Trinity Building, Pashan, Pune 411021, India
E-mail: srivatsan@iiserpune.ac.in

Abstract

Direct incorporation of azide groups into RNA oligonucleotides by in vitrotranscription reactions in the presence of a new azide-modified UTP analogue, and subsequent posttranscriptional chemical labeling of azide-modified oligoribonucleotide transcripts by click and Staudinger reactions are described. This postsynthetic labeling protocol is robust and modular, and offers an alternative access to RNA labeled with biophysical probes.

Graphical abstract: Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and Staudinger reactions

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1CC15659D
Article type
Communication
Submitted
13 Sep 2011
Accepted
05 Oct 2011
First published
17 Oct 2011

Chem. Commun., 2012,48, 498-500

Permissions

Request permissions

Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and Staudinger reactions

H. Rao, A. A. Sawant, A. A. Tanpure and S. G. Srivatsan, Chem. Commun., 2012, 48, 498 DOI: 10.1039/C1CC15659D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements