Issue 6, 2012

In situ monitoring of a trace intermediate during DNA phosphorylation by T4 polynucleotide kinase for transient kinetic studies

Abstract

Formation and decomposition of the enzyme–substrate (ES) complex during phosphorylation by T4 polynucleotide kinase (T4 PNK) of dsDNAs were monitored using a highly sensitive quartz crystal microbalance (QCM) to determine kinetic parameters, which were characterised in comparison with those of other enzymes such as DNA polymerase and exo- and endo-nucleases.

Graphical abstract: In situ monitoring of a trace intermediate during DNA phosphorylation by T4 polynucleotide kinase for transient kinetic studies

Supplementary files

Article information

Article type
Communication
Submitted
19 Dec 2011
Accepted
19 Jan 2012
First published
20 Jan 2012

Analyst, 2012,137, 1334-1337

In situ monitoring of a trace intermediate during DNA phosphorylation by T4 polynucleotide kinase for transient kinetic studies

H. Furusawa, K. Uemura, H. Yoshimine and Y. Okahata, Analyst, 2012, 137, 1334 DOI: 10.1039/C2AN16273C

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